can be separated from plasma to prepare packed cells which is used in
Severe anaemia. These can be stored in the refrigerator at 4 oC
to 6 oC till used.
These cells can also be frozen for 10 years at –18 oC in glycerol solution.
The Plasma is separated and frozen within 6 hrs. of phlebotomy and is called Fresh Frozen plasma (F.F.P) It is used for patients who have coagulation problems. FFP contains heat labile coagulation factor V and VIII-C. In some cases it is used for blood volume expander during surgery. It must be used within 24 hrs. of thawing or it must be used as a plain plasma (Single Donor Plasma) This SDP doesn’t contain heat labile coagulation factors. FFP may be stored for 1 year at –18 oC. After that it become plain plasma and may be stored for 5 years in the freezer. The fresh plasma is fractionated for the isolation of vital proteins in concentrate form.
Platelet rich plasma can be stored from 2-7 days from the day of phlebotomy, in A.C. room in the Blood bag containing anticoagulant preferably in a ‘Platelet Agitator’. If Platelet Concentrate is required then it should be infused to patient within 6 hrs. of preparation of the concentrate ideally. Platelet concentrate is achieved by pooling several unit of platelets into one bag.
Cryoprecipitate is factor VIII C which is also known as anti-haemophiliac factor. It is used for people who have Haemophilia. To prepare Cryprecipitate, FFP is thawed in a 1-6 oC water bath or Refrigerator within 24 hrs after it is frozen Cryprecipitate separates out into a form of white chalky substance in the FFP bag which can be separated with the use of cold centrifuge at 4 oC in satellite Bag. It can be stored for 1 yr. From the time of phlebotomy at –18 oC in.